Synthesis of hydrophobic insulin-based peptides using a helping hand strategy.
Disotuar, M.M., Petersen, M.E., Nogueira, J.M., Kay, M.S., Chou, D.H. (2018)
Org Biomol Chem, Jun 27 [Epub ahead of print]


Pharmacokinetic and chemical synthesis optimization of a potent D-peptide HIV entry inhibitor suitable for extended-release delivery. 
Redman, J.S., Francis, J.N., Marquardt, R., Papac, D., Mueller, A.L., Eckert, D.M., Welch, B.D., Kay, M.S. (2018)
Mol Pharm, 15: 1169-79.


Aligator: A computational tool for optimizing total chemical synthesis of large proteins. 
Jacobsen, M.T., Erickson, P.W., Kay, M.S. (2017) 
Bioorg Med Chem, 25: 4946-52.


Loquacious-PD facilitates Drosophila Dicer-2 cleavage through interactions with the helicase domain and dsRNA. 
Trettin, K.D., Sinha, N.K., Eckert, D.M., Apple, S.E., Bass, B.L. (2017) 
Proc Natl Acad Sci USA, 114: E7939-48.


A helping hand to overcome solubility challenges in chemical protein synthesis. 
Jacobsen, M.T., Petersen, M.E., Ye, X., Galibert, M., Lorimer, G.H., Aucagne, V., Kay, M.S. (2016) 
J Am Chem Soc, 138: 11775-82.


Synthesis of tumor necrosis factor α for use as a mirror-image phage display target. 
Petersen, M.E., Jacobsen, M.T., Kay, M.S. (2016) 
Org Biomol Chem, 14: 5298-303.


Construction of functional monomeric Type 2 Isopentenyl Diphosphate:Dimethylallyl Diphosphate Isomerase.
Neti, S.S., Eckert, D.M., Poulter, C.D. (2016)  
Biochemistry, 55: 4229-38.


Design and characterization of ebolavirus GP prehairpin intermediate mimics as drug targets. 
Clinton, T.R., Weinstock, M.T., Jacobsen, M.T., Szabo-Fresnais, N., Pandya, M.J., Whitby, F.G., Herbert, A.S., Prugar, L.I., McKinnon, R., Hill, C.P., Welch, B.D., Dye, J.M., Eckert, D.M., Kay, M.S. (2015) 
Protein Sci, 24: 446-63. 

2016 Protein Science Best Paper Award Winner


A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation. 
Zhao, H.,  Ghirlando, R., Alfonso, C.,  Arisaka, F., Attali, I., Bain, D.L., Bakhtina, M.M., Becker, D.F., Bedwell, G.J., Bekdemir, A., Besong, T.M.D., Birck, C., 12, Brautigam, C.A., Brennerman, W., Byron, O., Bzowska, A., Chaires, J.B., Chaton, C.T., Cölfen, H., Connaghan, K.D., Crowley, K.A., Curth, U., Daviter, T., Dean, W.L., Díez, A.I., Ebel, C., Eckert, D.M., Eisele, L.E., Eisenstein, E., England, P., Escalante, C., Fagan, J.A., Fairman, R., Finn, R.M., Fischle, W., García de la Torre, J., Gor, J., Gustafsson, H., Hall, D., Harding, S.E., Hernández Cifre, J.G., Herr, A.B., Howell, E.E., Isaac, R.S., Jao, S., Jose, D., Kim, S., Kokona, B., Kornblatt, J.A., Kosek, D., Krayukhina, E., Krzizike, D., Kusznir, E.A., Kwon, H., Larson, A., Laue, T.M., LeRoy, A., Leech, A.P., Lilie, H., Luger, K., Luque-Ortega, J.R., Ma, J., May, C.A., Maynard, E.L., Modrak-Wojcik, A., Mok, Y., Mücke, N.,  Nagel-Steger, L., Narlikar, G.J., Noda, M., Nourse, A., Obsil, T., Park, C.K., Park, J., Pawelek, P.D., Perdue, E.E., Perkins, S.J., Perugini, M.A., Peterson, C.L., Peverelli, M.G., Piszczek, G., Prag, G., Prevelige, P.E., Raynal, B.D.E., Rezabkova, L., Richter, K., Ringel, A.E., Rosenberg, R., Rowe, A.J., Rufer, A.C., Scott, D.J., Seravalli, J.G., Solovyova, A.S., Song, R., Staunton, D., Stoddard, C., Stott, K., Strauss, H.M., Streicher, W.W., Sumida, J.P., Swygert, S.G., Szczepanowski, R.H., Tessmer, I., Toth IV, R.T., Tripathy, A., Uchiyama, S., Uebel, S.F.W., Unzai, S., Vitlin Gruber, A., von Hippel, P.H., Wandrey, C., Wang, S., Weitzel, S.E., Wielgus-Kutrowska, B., Wolberger, C., Wolff, M., Wright, E., Wu, Y., Wubben, J.M., Schuck, P.* (2015) 
PLoS One, 10(5), e0126420.


Synthesis and folding of a mirror-image enzyme reveals ambidextrous chaperone activity. 
Weinstock, M.T., Jacobsen, M.T. and Kay, M.S. (2014) 
Proc Natl Acad Sci USA, 111: 11679-84.


A phosphatidylinositol transfer protein integrates phosphoinositide signaling with lipid droplet metabolism to regulate a developmental program of nutrient stress-induced membrane biogenesis. 
Ren, J., Pei-Chen Lin, C., Pathak, M.C., Temple, B.R., Nile, A.H., Mousley, C.J., Duncan, M.C., Eckert, D.M., Leiker, T.J., Ivanova, P.T., Myers, D.S., Murphy, R.C., Brown, H.A.,Verdaasdonk, J., Bloom, K.S., Ortlund, E.A., Neiman, A.M., and Bankaitis, V.A. (2014) 
Mol Biol Cell, 25: 712-27.


The oligomeric state of the active Vps4 AAA ATPase. 
Monroe, N., Han, H., Gonciarz, M.D., Eckert, D.M., Karren, M.A., Whitby, F.G., Sundquist, W.I.,  and Hill, C.P. (2014) 
J Mol Biol, 426: 510-25.


Novel preclinical models of topical PrEP pharmacodynamics provide rationale for combination of drugs with complementary properties. 
Mesquita, P.M., Srinivasan, P., Johnson, T.J., Rastogi, R., Evans-Strickfaden, T., Kay, M.S., Buckheit, K.W., Buckheit, R.W. Jr., Smith, J.M., Kiser, P.F. and Herold, B.C. (2013) 
Retrovirol, 10: 113.


Interchangeable adaptors regulate mitochondrial dynamin assembly for membrane scission.
Koirala, S., Guo, Q., Kalia, R., Bui, H.T., Eckert, D.M., Frost, A., and Shaw, J.M. (2013) 
Proc Natl Acad Sci USA, 110: E1342-51.


Virion stiffness regulates immature HIV-1 entry. 
Pang, H.B., Hevroni, L., Kol, N., Eckert, D.M., Tsvitov, M., Kay, M.S., and Rousso, I. (2013)
Retrovirol, 10: 4.


Protease-resistant peptide design – empowering nature’s fragile warriors against HIV. 
Weinstock, M.T., Francis, J.N., Redman, J.S., and Kay, M.S. (2012) 
Peptide Sci, 98: 431-42.


Structure of a proteasome Pba1-Pba2 complex: implications for proteasome assembly, activation, and biological function. 
Stadtmueller, B.M., Kish-Trier, E., Ferrell, K., Petersen, C.N., Robinson, H., Myszka, D.G., Eckert, D.M., Formosa, T., and Hill C.P. (2012) 
J Biol Chem, 287: 37371-82.


Design of a modular tetrameric scaffold for the synthesis of membrane-localized D-peptide inhibitors of HIV-1 entry.  
Francis, J.N., Redman, J.S., Eckert, D.M. and Kay, M.S. (2012) 
Bioconjugate Chem, 23: 1252-8.


Revealing the allosterome: systematic identification of metabolite-protein interactions. 
Orsak, T., Smith, T.L., Eckert, D.M., Lindsley, J.E., and Rutter, J. (2011) 
Biochem, 51: 225-32.


One percent tenofovir applied topically to humanized BLT mice and used according to the CAPRISA 004 experimental design demonstrates partial protection from vaginal HIV infection, validating the BLT model for evaluation of new microbicide candidates. 
Denton, P.W., Othieno, F., Martinez-Torres, F., Zou, W., Krisko, J.F., Fleming, E., Zein, S., Powell, D.A., Wahl, A, Kwak, Y.T., Welch, B.D., Kay, M.S., Payne, D.A., Gallay, P., Appella, E., Estes, J.D., Lu, M. and Garcia, J.V. (2011) 
J Virol, 85: 7582-93.


Design of a potent D-peptide HIV-1 entry inhibitor with a strong barrier to resistance. 
Welch, B.D., Francis, J.N., Redman, J.S., Paul, S., Weinstock, M.T., Reeves, J.D., Lie, Y.S., Whitby F.G., Eckert, D.M., Hill, C.P., Root, M.J., and Kay, M.S. (2010) 
J Virol, 84: 11235-44.


The effect of purification method on the completeness of the immature HIV-1 Gag shell. 
Kol N., Tsvitov M., Hevroni L., Wolf S.G., Pang H.B., Kay M.S., and Rousso I. (2010) 
J Virol Methods, 169: 244-7.


Stalking influenza. 
Eckert, D.M. and Kay, M.S. (2010) 
Proc Natl Acad Sci USA, 107: 13563-4.


Molecular architecture of a dynamin adaptor: implications for assembly of mitochondrial fission complexes. 
Koirala, S., Bui, H.T., Schubert, H.L., Eckert, D.M., Hill, C.P., Kay, M.S. and Shaw, J.M. (2010)
J Cell Biol, 191: 1127-39.


Structural and functional studies on the extracellular domain of BST2/tetherin in reduced and oxidized conformations. 
Schubert, H.L., Zhai, Q., Sandrin, V., Eckert, D.M., Garcia-Maya, M., Saul, L., Sundquist, W.I., Steiner, R.A., and Hill, C.P. (2010) 
Proc Natl Acad Sci USA, 107: 17951-6.


Structural basis for ESCRT-III protein autoinhibition. 
Bajorek, M., Schubert, H.L., McCullough, J., Langelier, C., Eckert, D.M., Stubblefield, W-M.B., Uter, N.T., Hill, C.P., and Sundquist, W.I. (2009) 
Nat Struct Mol Biol, 16: 754-62.


Peptide mimic of the HIV envelope gp120-gp41 interface. 
Kim, S., Pang, H.B., and Kay M.S. (2008) 
J Mol Biol, 376: 786-97.


Characterization of the steric defense of the HIV-1 gp41 N-trimer region. 
Eckert, D.M., Shi, Y., Kim, S., Welch, B.D., Kang, E., Poff, E.S., and Kay, M.S. (2008)
Protein Sci, 17: 2091-100.


Biochemical and structural studies of yeast Vps4 oligomerization. 
Gonciarz, M.D., Whitby, F.G., Eckert, D.M., Kieffer, C., Heroux, A., Sundquist, W.I., and Hill, C.P. (2008) 
J Mol Biol, 384: 878-95.


Biochemical characterization of a recombinant TRIM5α protein that restricts HIV-1 replication. 
Langelier, C.R., Sandrin, V., Eckert, D.M., Christensen, D.E., Chandrasekaran, V., Alam, S.L., Aiken, C., Olson, J.C., Kar, A.K., Sodroski, J.G., and Sundquist, W.I. (2008) 
J Virol, 82: 11682-94.


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Potent D-peptide inhibitors of HIV-1 entry. 
Welch, B.W., VanDemark, A.P., Hill, C.P., and Kay, M.S. (2007) 
Proc Natl Acad Sci USA, 104: 16828-33.


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A stiffness switch in human immunodeficiency virus. 
Kol, N., Shi, Y., Tsvitov, M., Barlam, D., Shneck, R.Z., Kay, M.S. & Rousso, I. (2007) 
Biophys J, 92: 1777-83.


Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding. 
Morita, E., Sandrin, V., Alam, S.L., Eckert, D.M., Gygi, S.P. and Sundquist, W.I. (2007)
Cell Host Microbe, 2: 41-53.


RDE-4 preferentially binds long dsRNA and its dimerization is necessary for cleavage of dsRNA to siRNA. 
Parker, G.S., Eckert D.M., and Bass, B.L. (2006) 
RNA, 12: 807-18.


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Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1. 
Azmi, I., Davies, B., Dimaano, C., Payne, J., Eckert D., Babst, M., and Katzmann, D.J. (2006)
J Cell Biol, 172: 705-17.


Steric accessibility of the HIV-1 gp41 N-trimer region. 
Hamburger, A.H., Kim, S., Welch, B.D., Kay M.S. (2005) 
J Biol Chem, 280: 12567-72.


Inhibiting HIV fusion with a ß-peptide foldamer. 
Stephens, O.M., Kim, S., Welch, B.D., Hodsdon, M.E., Kay, M.S., Schepartz, A. (2005)
J Am Chem Soc, 127: 13126-7.


Silent, but deadly—eliminating reservoirs of latent HIV.
Kay, M.S.
(2003)
Trends Biotechnol, 21: 420-3.


Protein design of an HIV-1 entry inhibitor. 
Root, M.J., Kay, M.S., and Kim, P.S. (2001) 
Science, 291: 884-8.


Design of potent inhibitors of HIV-1 entry from the gp41 N-peptide region. 
Eckert, D.M. and Kim, P.S. (2001) 
Proc Natl Acad Sci USA, 98: 11187-92.


Mechanisms of viral membrane fusion and its inhibition. 
Eckert, D.M. and Kim, P.S. (2001) 
Annu Rev Biochem, 70: 777-810.


Inhibiting HIV-1 entry: Discovery of D-peptide inhibitors that target the gp41 coiled-coil pocket. 
Eckert, D.M., Malashkevich, V.N., Hong, L.H., Carr, P.A., and Kim, P.S. (1999) 
Cell, 99: 103-15.